• Membrane Adsorber

    for biomolecule purification with i3 Digital Membrane Chromatography

    The i3 Digital Membrane Chromatography Adsorber is designed for the efficient and gentle purification of biomolecules using the DMC-elution method.

    • Ideal for rapid capture and release of highly concentrated biomolecules, monoclonal antibodies and Fc-containing molecules

    • Enables work in small-scale applications such as research, screening and process development
    i3 DMC AEX i3 DMC CEX i3 DMC PrA

    Its charged surface enhances the binding and separation process, providing rapid purification without compromising the integrity of your samples via DMC. Featuring an integrated ultra-thin gold membrane and contact pins on the housing, this advanced design allows for precise application of an electric field via the i3 DMControl Smart Control Unit. This ensures optimal control over the elution process, enhancing both performance and consistency.

    The Membrane Adsorber is equipped with Luer-Lock connectors for seamless integration with liquid chromatography (LC) systems, making it easy to incorporate into your existing workflows for maximum convenience and flexibility.

    i3 DMC AEX

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    The i3 DMCAEX is intended for the purification of negatively charged biomolecules using electrical potential for elution. Digital Membrane Chromatography (DMC) enables a rapid purification of highly concentrated eluates, ideal for drug discovery applications and the production of biomolecules for research and development purposes. The Anion Exchange Membrane Adsorber is equipped with four pins enabling the electric potential to be precisely directed onto the membrane. It can be easily integrated into a liquid chromatography system using Luer-Lock connection.

    • Rapid and gentle purification of negatively charged biomolecules

    • Ideal for small-scale application, e.g. research and process development

    • Highly concentrated eluates without aggregation

    • No buffer exchannge

    • Easy connection to penstaltic pump or chromatography systems
    Membrane Polyamide (PA)
    Pore size 0.2 µm
    Effective filtration area Ø 22.0 mm | Ø 0.89″
    Membrane thickness 0.11 mm | 0.004″
    Bed volume 0.04 mL
    Dynamic binding capacity per unit 0.6 mg BSA (Determined in 0.24 mM PBS, pH 7.4)
    Recommended operating flow rate 2.0 mL/min
    Maximum operating pressure 3 bar | 43.5 psi
    Recommended elution voltage – 2 V
    Temperature stability 4°C-RT @ 39.2°F-RT
    Connectors Luer-Lock
    Autoclavability non autoclavable
    Dimensions H: 34.0 mm | 1.3″
    Ø: 29.7 mm | 1.2″

    i3 DMC CEX

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    The i3 DMCCEX is intended for the purification of positively charged biomolecules
    using electric potential for elution. It enables a rapid purification of highly concentrated eluates for drug discovery applications and in the productions of biomolecules for research and development.

    • Rapid and gentle purification on negatively charged biomolecules

    • Ideal for small-scale application, e.g. research and process development

    • Highly concentrated eluates without aggregation through multiple elution cycles

    • No buffer exchange
    Membrane Polyamide (PA)
    Pore size 0.2 µm
    Effective filtration area 22.0 mm | 0.89″
    Membrane thickness 0.11 mm | 0.004″
    Bed volume 0.04 mL
    Dynamic binding capacity per unit 1.1 mg Lysozyme (Determined in 0.24 mM PBS, pH 7.4)
    Recommended operating flow rate 2.0 mL/min
    Maximum operating pressure 3 bar | 43.5 psi
    Recommended elution voltage + 2 V
    Temperature stability 4°C-RT @ 39.2°F-RT
    Connectors Luer-Lock
    Autoclavability non autoclavable
    Dimensions H: 34.0 mm | 1.3″
    Ø: 29.7 mm | 1.2″

    i3 DMC PrA

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    The i3 DMCPrA is intended for the affinity purification of monoclonal antibodies and Fc-containing molecules using electrical potential for elution. It enables a rapid purification of highly concentrated eluates for drug discovery applications and in the productions of biomolecules for research and development.

    • Rapid and gentle purification on mAbs and Fc-containing proteins

    • Highly concentrated eluates without aggreration through multiple elution cycles

    • Easy connection to peristatic pumps or chromatographic systems

    • No buffer exchange
    Membrane Cellulose (CEL)
    Pore size 0.2 µm
    Effective membrane diameter 22.0 mm | 0.89″
    Membrane thickness 0.25 mm ± 30 µm ǀ 0.01” ± 0.0012“
    Bed volume 0.1 mL
    Dynamic binding capacity per unit* up to 5.1 mg IgG1
    Recommended operating flow rate 2.0 mL/min
    Maximum operating pressure 3 bar | 43.5 psi
    Recommended elution voltage + 2 V
    Temperature stability 4°C-RT @ 39.2°F-RT
    Connectors Luer-Lock
    Autoclavability non autoclavable
    Dimensions H: 34.0 mm | 1.3″
    Ø: 29.7 mm | 1.2″

    *determined in 0.24 mM PBS**, pH 7.4
    **i3 standard PBS working solution: 0.24 mM phosphate buffer, 2.74 mM NaCl and 0.054 mM KCl