Membrane Adsorber

Digital Ion-Exchange Membrane Chromatography for the Targeted Purification of Charged Biomolecules

i3 DMC AEX and CEX combine the charge-based separation of ion-exchange chromatography with the electrically controlled elution of digital membrane chromatography (DMC). Depending on the charge, either AEX or CEX selectively binds the target molecule to the membrane. In conjunction with the smart control unit, the bound molecules are released via a controlled electrical potential, rather than through conventional elution using high salt concentrations.

Electric Instead of Salt-Based
A controlled electric voltage releases bound molecules without the need for high salt concentrations
Targeted Binding
AEX binds negatively charged biomolecules, while CEX binds positively charged biomolecules
A ready-to-analyze sample in 10 minutes
No more neutralization or buffer exchange required
Efficient Recovery
Targeted electrical release enables high recovery of bound target molecules

i3 DMC CEX i3 DMC AEX

Transforming Purification with i3 DMC Ion Exchange Chromatography

Ion exchange chromatography uses electrostatic interactions to selectively bind and separate biomolecules based on their charge. With digital membrane chromatography, i3 Membrane expands this proven principle to include a new form of process control: While traditional ion-exchange methods require high salt concentrations or altered pH conditions for elution, DMC makes the release of bound molecules electrically controllable.

To achieve this, the chromatographic membrane is positioned between two gold-coated electrode membranes and connected to the smart control unit via integrated contacts. Through these contacts, a predefined low voltage is applied to the membrane adsorber, thereby altering local electrochemical conditions within the membrane system. These changes influence the charge-based interaction between the membrane and the target molecule, enabling its release. Elution is thus activated precisely where the binding occurs: directly on the membrane.

AEX and CEX offer two complementary separation options: AEX binds negatively charged biomolecules, while CEX binds positively charged biomolecules. The membrane adsorbers handle the targeted binding and separation, while the i3 DMC Control manages the electrical elution step. Together, they form the DMC system and can be intuitively integrated into existing FPLC systems via Luer-Lock connections. The elimination of high salt concentrations and the need for additional buffer exchange reduces the number of process steps and enables rapid, concentrated eluates for downstream analyses and early-stage process development.

i3 DMC CEX

Request Information

The i3 DMC CEX is designed for the purification of positively charged biomolecules using cation exchange. Negatively charged functional groups on the open pore membrane enable selective binding under suitable pH conditions. This allows proteins and other biomolecules to be selectively captured based on their charge and subsequently released through electrically driven elution.

Product Highlights

Selective binding of positively charged biomolecules
Negatively charged functional groups for cation binding
Open pore membrane for rapid molecular binding
Controlled release through applied voltage

Applications

Purification of positively charged proteins and other biomolecules
Protein separation and sample preparation for analytical workflows

Membrane	Polyamide (PA)
Pore size	0.2 µm
Effective filtration area	Ø 22.0 mm \| Ø 0.89″
Membrane thickness	0.11 mm \| 0.004″
Bed volume	0.04 mL
Dynamic binding capacity per unit	0.6 mg BSA (Determined in 0.24 mM PBS, pH 7.4)
Recommended operating flow rate	2.0 mL/min
Maximum operating pressure	3 bar \| 43.5 psi
Recommended elution voltage	– 2 V
Temperature stability	4°C-RT @ 39.2°F-RT
Connectors	Luer-Lock
Autoclavability	non autoclavable
Dimensions	H: 34.0 mm \| 1.3″ Ø: 29.7 mm \| 1.2″

i3 DMC AEX

Request Information

The i3 DMC AEX is an anion exchange membrane adsorber for the selective purification of negatively charged biomolecules. Positively charged functional groups on the open pore membrane enable selective binding based on the charge of the molecule and the selected buffer conditions. Convective flow transports the molecules directly to the binding sites and supports rapid charge based separation.

Produkt Highlights:

Selective binding of negatively charged biomolecules
Positively charged functional groups for anion binding
Open pore membrane for convective mass transport
Electrically driven elution without high salt concentrations

Anwendungen:

Purification of negatively charged proteins and other biomolecules
Charge based separation in research, screening and analytical sample preparation

Membrane	Polyamide (PA)
Pore size	0.2 µm
Effective filtration area	Ø 22.0 mm \| Ø 0.89″
Membrane thickness	0.11 mm \| 0.004″
Bed volume	0.04 mL
Dynamic binding capacity per unit	0.6 mg BSA (Determined in 0.24 mM PBS, pH 7.4)
Recommended operating flow rate	2.0 mL/min
Maximum operating pressure	3 bar \| 43.5 psi
Recommended elution voltage	– 2 V
Temperature stability	4°C-RT @ 39.2°F-RT
Connectors	Luer-Lock
Autoclavability	non autoclavable
Dimensions	H: 34.0 mm \| 1.3″ Ø: 29.7 mm \| 1.2″

How the i3 DMC Workflow Works

Integrating the Membrane Adsorber
The selected i3 DMC membrane adsorber is integrated into the FPLC system’s fluid path via Luer-Lock connections
Make the electrical connection
The connection cables of the i3 DMC Control are connected to the integrated IN and OUT contacts of the membrane adsorber
Select the protocol
Depending on the membrane adsorber, format, and application, a single voltage, a preset, or a custom sequence is selected
Trigger elution
During the purification process, the i3 DMC Control applies the defined voltage at the appropriate time. The resulting local electrochemical reactions lead to the release of the bound target molecules
Process the eluate
The FPLC system removes the eluate from the membrane adsorber and makes it available for further processing or downstream analysis

Order your sample pack now!

Contact our DMC experts and request samples of our i3 Digital Membrane Chromatography.

Request a sample now