Membrane Adsorber

Digital Protein A Membrane Chromatography for the Purification of Monoclonal Antibodies, Fc-Containing Molecules, and Next-Generation Antibody Formats

i3 DMC PrA combines Protein A affinity with Digital Membrane Chromatography (DMC) for the selective and mild purification of even sensitive antibodies. In conjunction with the smart control unit, DMC selectively releases the bound target molecules using a controlled electrical voltage, eliminating the need for conventional low-pH elution buffers.

Mild Elution
Elution using an electric voltage minimizes the risk of pH-induced aggregation
Up to 90% recovery
Efficient use of valuable samples, even in limited quantities
Analysis-ready in under 10 min.
Fast workflow without neutralization or buffer exchange
Easy Integration
Connect to existing FPLC systems using the Luer-Lock connector

i3 DMC PrA 0.1 mL i3 DMC PrA 1.0 mL

Transforming Purification with i3 DMC Affinity Chromatography

Affinity chromatography is one of the most selective methods for purifying antibodies and other biomolecules. With Digital Membrane Chromatography (DMC), i3 Membrane expands this proven principle to include a new form of process control: While traditional Protein A methods require a switch to acidic buffer conditions for elution, DMC makes the release of bound molecules electrically controllable.

To achieve this, the membrane is positioned between two gold-coated electrode membranes and connected via integrated contacts to the digital control unit, the i3 DMC Control. The control unit applies a voltage to the membrane adsorbent, thereby altering local electrochemical conditions within the membrane system. These locally generated changes influence the interaction between the membrane and the target molecule, enabling its release. Elution is thus activated precisely where the binding occurs – directly on the membrane.

This opens up new possibilities for sample preparation in early discovery and preclinical research. The technology reduces harsh process conditions, helps preserve protein integrity, and makes samples available more quickly for downstream analyses. In this way, i3 DMC addresses a key challenge in modern antibody research: Rapid purification with maximum recovery rates, without acid-induced aggregation or fragmentation. This creates a new approach for fast, precise, and mild purification processes in modern biotech workflows.

i3 DMC PrA 0.1 mL

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The i3 DMC PrA 0.1 mL is a Protein A-functionalized membrane adsorber for the selective purification of monoclonal antibodies, Fc-containing molecules, and other Protein A-binding molecules. Its two-layer, open-pore cellulose membrane (0.2 µm) allows for convective flow and transports target molecules directly to the binding sites. With a bed volume of 0.1 mL and an IgG1 binding capacity of up to 5 mg, the adsorber is suitable for use in research and development applications.

Product Highlights:

Mild, electrically controlled elution without acidic pH conditions
Preservation of the native antibody structure without aggregation or fragmentation
Enables direct analytical analysis
Neutralization and buffer exchange are no longer necessary

Applications:

Antibody purification from cell culture supernatants and other complex sample matrices
Sample preparation for mass spectrometry and other analytical methods
Can be used for up to ten purification cycles with a suitable CIP protocol

Membrane	Cellulose (CEL)
Pore size	0.2 µm
Effective membrane diameter	22.0 mm \| 0.89″
Membrane thickness	0.25 mm ± 30 µm ǀ 0.01” ± 0.0012“
Bed volume	0.1 mL
Dynamic binding capacity per unit*	Up to 5.1 mg IgG1
Recommended operating flow rate	2.0 mL/min
Maximum operating pressure	3 bar \| 43.5 psi
Recommended elution voltage	+ 2 V
Temperature stability	4°C-RT @ 39.2°F-RT
Connectors	Luer-Lock
Autoclavability	Non autoclavable
Dimensions	H: 34.0 mm \| 1.3″ Ø: 29.7 mm \| 1.2″

*determined in 0.24 mM PBS**, pH 7.4
**i3 standard PBS working solution: 0.24 mM phosphate buffer, 2.74 mM NaCl and 0.054 mM KCl

i3 DMC PrA 1.0 mL NEW

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The i3 DMC PrA 1.0 mL is a ready-to-use Protein A membrane adsorber with mild, electrically driven elution for the rapid affinity purification of IgG, mAbs, and other Fc-containing molecules. Its Protein A-functionalized cellulose membrane combines a 1.0 mL bed volume with a dynamic binding capacity of up to 30 mg of polyclonal IgG, providing more purified material for extended analytical characterization and early development. The new format brings the established i3 DMC approach to larger-scale purification while retaining the same fundamental separation principle and workflow, creating a scalable solution as project requirements grow.

Product Highlights:

1.0 mL bed volume supports larger purification workflows
Up to 30 mg polyclonal IgG binding capacity
Mild, electrically driven elution without low-pH buffers
Maximum yield from low-abundance sample with recovery rates of 90%

Applications:

Preparation for early in vivo pharmacology studies
Supports mass spectrometry, ELISA, BLI, and SPA workflows.
Up to ten purification cycles with suitable CIP

Membrane material	Cellulose
Ligand	Protein A
Pore size	0,2 µm
Membrane dimensions	76.5 x 32.5 mm
Membrane thickness	0.625 mm ± 30 µm \| 0.025” ± 0.0012”
Bed volume (BV)/Membrane volume (MV)	1.0 mL
Dynamic binding capacity (DBC)/unit¹	Up to 30 mg polyclonal IgG
Elution volume	8 mL (8 MV)
Recommended operating flow rate	3.0 mL/min (3 MV/min)
Maximum operating pressure	Up to 5 bar \| 72.5 psi
Recommended elution voltage	+3.35 V
Temperature stability	4°C – RT \| 39.2°F – RT
Cleaning-in-place (CIP)	Up to 10x with 0.1 M NaOH, short-time incubation
Connectors	PEEK Finger tight Fitting, 10-32 Thread
Autoclavability	Non autoclavable
Storage	20% ethanol/water, 4°C \| 39.2°F
Dimensions	L: 130.0 mm \| 5.1” W: 86.0 mm \| 3.4” H: 28.0 mm \| 1.1″

¹Determined in 1:50 diluted 1x PBS (containing 2.74 mM NaCl, 54 µM KCl, 0.24 mM phosphate buffer, pH 7.4)

How the i3 DMC Workflow Works

Integrating the Membrane Adsorber
The selected i3 DMC membrane adsorber is integrated into the FPLC system’s fluid path via Luer-Lock connections
Make the electrical connection
The connection cables of the i3 DMC Control are connected to the integrated IN and OUT contacts of the membrane adsorber
Select the protocol
Depending on the membrane adsorber, format, and application, a single voltage, a preset, or a custom sequence is selected
Trigger elution
During the purification process, the i3 DMC Control applies the defined voltage at the appropriate time. The resulting local electrochemical reactions lead to the release of the bound target molecules
Process the eluate
The FPLC system removes the eluate from the membrane adsorber and makes it available for further processing or downstream analysis

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